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KMID : 0380020190340030199
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Korean Journal of Biotechnology and Bioengineering
2019 Volume.34 No. 3 p.199 ~ p.206
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Adequacy of the Anti-aging and Anti-wrinkle Effects of the Artemisia vulgaris Fermented Solvent Fraction
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Park Ye-Seul
Nam Gun-He
Jo Kyung-Jo
Kawk Hye-Won
Yoo Je-Geun
Jang Jin-Dong
Kang Sang-Moon
Kim Sang-Yong
Kim Young-Min
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Abstract
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Collagen is decomposed by MMP-1, an enzyme induced by transcription factor activator protein 1 (AP-1) and matrix metalloproteinase-9 (MMP-9). Action of MMPs in inflammatory response promotes inflammatory cell movement and secretion, resulting in wrinkles on the skin. After using Bacillus sp. fermentation system and water, Artemisia vulgaris was fermented to prepare different solvent fractions using water, dichloromethane, hex ane, n-butanol, and ethyl acetate. These fractions were used to assess their effects on cell survival, wound healing, MMP-1/MMP-9 and procollagen type I C-peptide (PICP) expression, and skin turnover. MTT assay showed that cell viability of each treated group was 103% to 121%, indicating that A. vulgaris fractions were not toxic compared to control (cell viability: 100%). Wound healing assay revealed that wound healing ability in each treated group was 51% to 61%. This was lower than wound healing area in the control. Using RT-PCR, inhibition of MMP-1/MMP-9 gene expression was examined. As a result, each group treated with fraction showed reduced expression of both MMP-1 and MMP-9 compared to tumor necrosis factor- α (TNF-α) treatment group. Effects on collagen biosynthesis were analyzed using a PICP ELISA Kit. The group in which Artemisia vulgaris was treated increased collagen synthesis from 141 to 262ng/mL compared to the control group. Three-dimensional cell culture revealed that each fraction could increase skin wall formation. These results suggest that each fractions has anti-aging and anti-wrinkle effects on the skin, indicating its suitability as a functional material.
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KEYWORD
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anti-aging, anti-wrinkle, Artemisia vulgaris fermented solvent fraction, MMP-1/MMP-9, procollagen
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